REVIEW PAPER
Cracking the Metabolic engineering of bacteria: Review of methods involved in organic acid Production
Manam Walait 1  
,   Huda Rehman mir 1  
,   Zainab Hassan 1  
,   Javed Iqbal Wattoo 1  
 
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Department of Biotechnology, Faculty of Life Sciences, University of Central Punjab, Lahore, Pakistan
CORRESPONDING AUTHOR
Manam Walait   

Department of Biotechnology, Faculty of Life Sciences, University of Central Punjab, Lahore, Pakistan
Submission date: 2021-09-02
Final revision date: 2021-10-27
Acceptance date: 2021-11-02
Online publication date: 2021-11-08
 
 
KEYWORDS
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ABSTRACT
Metabolic engineering is defined as recombinant DNA technology to improve specific biochemical reactions for product formation. We modify the metabolic processes of bacteria to get our desired food by metabolic engineering. Metabolic engineering will enhance these microorganisms' properties and their ability to produce a diverse number of products cost-effectively. To produce amino acids, we modify the central metabolic pathway, biosynthetic pathway, and transport pathway. In many food industries, the production of organic acids through different processes and techniques have proved very beneficial because of their widespread applications. In line with this information, the present review aimed to provide background information for researchers about genetically modified foods for increased food yield to fulfil the nutritional values for average body growth.
 
REFERENCES (54)
1.
Agbor-Egbe, T., Mbome, I.L.J.J.O.F.C., 2006. The effects of processing techniques in reducing cyanogen levels during the production of some Cameroonian cassava foods. Journal of Food Composition and Analysis. 19, 354–363. https://doi.org/10.1016/j.jfca....
 
2.
Alkay, Z., Dertli, E., Durak, M.J.A.A., 2021. Investigation of probiotic potential of yeasts isolated from sourdoughs from different regions of Turkey. Acta Alimentaria. https://doi.org/10.1556/066.20....
 
3.
Annunziato, G., Spadini, C., Franko, N., Storici, P., Demitri, N., Pieroni, M., Flisi, S., Rosati, L., Iannarelli, M., Marchetti, M.J.A.I.D., 2021. Investigational Studies on a Hit Compound Cyclopropane-Carboxylic Acid Derivative Targeting O-Acetylserine Sulfhydrylase as a Colistin Adjuvant. ACS Infectious Diseases. 7, 281–292. https://doi.org/10.1021/acsinf....
 
4.
Antoniewicz, M.R.J.M.E., 2020. A guide to metabolic flux analysis in metabolic engineering: Methods, tools and applications. Metabolic Engineering. 63, 2–12. https://doi.org/10.1016/j.ymbe....
 
5.
Bar-Even, A., Flamholz, A., Noor, E., Milo, R.J.N.C.B., 2012. Rethinking glycolysis: on the biochemical logic of metabolic pathways. Nature Chemical Biology. 8, 509–517. https://doi.org/10.1038/nchemb....
 
6.
Benthin, S., Villadsen, J.J.A.M., Biotechnology., 1995. Production of optically pure D-lactate by Lactobacillus bulgaricus and purification by crystallisation and liquid/liquid extraction. Applied Microbi- ology and Biotechnology. 42, 826–829. https://doi.org/10.1007/ BF00191176.
 
7.
Boels, I.C., Kranenburg, R.V., Hugenholtz, J., Kleerebezem, M., Vos, W.M.J.I.D.J.D., 2001. Sugar catabolism and its impact on the biosynthesis and engineering of exopolysaccharide production in lactic acid bacteria. International Dairy Journal. 11, 723–732. https:// doi.org/10.1016/S0958-6946(01)....
 
8.
Causey, T., Zhou, S., Shanmugam, K., J, L., 2003. Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: Homoacetate production. Proceedings of the National Academy of Sciences. 100, 825–832. https://doi.org/10.1073/pnas.0....
 
9.
Chang, D.E., Jung, H.C., Rhee, J.S., J, J.-G., 1999. Homofermentative production of D- or L-lactate in metabolically engineered Escherichia coli RR1. Applied and Environmental Microbiology. 65, 1384–1389. https://doi.org/10.1128/aem.65....
 
10.
Chotani, G., Dodge, T., Hsu, A., Kumar, M., Laduca, R., Trimbur, D., Weyler, W., B, K.J.B.E., Sanford, A.-P.S., Enzymology, M., 2000. The commercial production of chemicals using pathway engineering. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1543, 434–455. https://doi.org/10.1016/ S0167-4838(00)00234-X.
 
11.
Chowdhury, N., Yasmin, J., 2018. A study on sustainable manufacturing practices in RMG industries of Bangladesh. Munich Personal RePEc Archive. 87104, 1–17.
 
12.
Clark, D.P.J.F., 1989. The fermentation pathways of Escherichia coli. FEMS Microbiology Reviews. 5, 223–234. https://doi.org/10.1016/0168-6....
 
13.
Ferreira, I.M., Guido, L.F.J.F., 2018. Impact of wort amino acids on beer flavour: A review. Fermentation. 4(2), 23. https://doi.org/10.3390/ fermentation4020023.
 
14.
Friedman, C., Shagina, L., Lussier, Y., Hripcsak, G.J.J.O.T.A.M.I.A., 2004. Automated encoding of clinical documents based on natural language processing. Automated encoding of clinical documents based on natural language processing. 11, 392–402. https://doi.org/10.1197/jamia.....
 
15.
Gokarn, R., Eiteman, M., Altman, E.J.B., 1998. Expression of Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli without Affecting Glucose Uptake. Biotechnology Letters. 20, 795– 798. https://doi.org/10.1023/B:BILE....
 
16.
Gonzalez, R., Andrews, B.A., Molitor, J., Asenjo, J.A.J.B., Bioengi- neering., 2003. Metabolic analysis of the synthesis of high levels of intracellular human SOD in Saccharomyces cerevisiae rhSOD 2060 411 SGA122. Biotechnology & Bioengineering. 82, 152–169. https://doi.org/10.1002/bit.10....
 
17.
Hanlon, P., Sewalt, V., 2021. _genetically engineered microorganisms and the regulatory oversight of their uses in modern food production. Critical Reviews in Food Science and Nutrition. 61, 959–970. https:// doi.org/10.1080/10408398.2020.....
 
18.
Hao, T., Han, B., Ma, H., Fu, J., Wang, H., Wang, Z., Tang, B., Chen, T., Zhao, X.J.M.B., 2013. In silico metabolic engineering of Bacillus subtilis for improved production of riboflavin, Egl-237, (R,R)-2,3- butanediol and isobutanol. Molecular BioSystems. 9, 2034–2044. https://doi.org/10.1039/c3mb25....
 
19.
Hoet, P., Stainer, R.J.E., 1970. Existence and functions of two enzymes with beta-ketoadipate: succinyl-CoA transferase activity in Pseudomonas florescens. European Journal of Biochemistry. 13, 71– 76. https://doi.org/10.1111/j.1432....
 
20.
Holms, W., 1987. Control of flux through the citric acid cycle and the glyoxylate bypass in Escherichia coli. Biochemical Society symposium. 54, 17–31.
 
21.
Ikemi, M.J.R.M.F.I., 1994. Industrial chemicals: enzymatic transformation by recombinant microbes. Bioprocess Technology. 19, 797–813. Ivanov, K., Stoimenova, A., Obreshkova, D., Saso, L.J.B., Equipment, B., 2013. Biotechnology in the Production of Pharmaceutical Industry Ingredients: Amino Acids. Biotechnology & Biotechnological Equipment. 27, 3620–3626. https://doi.org/10.5504/BBEQ.2....
 
22.
Leblanc, J.G., Taranto, M.P., Molina, V., J, F., 2010. B-Group Vitamins Production by Probiotic Lactic Acid Bacteria, In: Second (Eds.); F. Mozzi, R.R. Raya, G.M. Vignolo, et al., (Eds.), Biotechnology of Lactic Acid Bacteria: Novel Applications. John Wiley & Sons, Ltd, pp. 211–232. https://doi.org/10.1002/978111....
 
23.
Leonard, E., Lim, K.H., Saw, P.N., Koffas, M.A.J.A., Microbiology, E., 2007. Engineering central metabolic pathways for high-level flavonoid production in Escherichia coli. Applied and Environmental Microbiology. 73, 3877–3886. https://doi.org/10.1128/AEM.00....
 
24.
Levine, M., Rumsey, S.C., Daruwala, R., Park, J.B., Wang, Y.J.J., 1999. Criteria and recommendations for vitamin C intake. JAMA. 281, 1415–1423. https://doi.org/10.1001/jama.2....
 
25.
Lixin, S.J.J.O.M., 2000. Metabolic engineering: A rising filed in biochemical engineering. Journal of Microbiology. 3.
 
26.
Longo, M.A., Sanromán, M.A.J.F.T., Biotechnology., 2006. Production of Food Aroma Compounds: Microbial and Enzymatic Methodologies. Food Technology and Biotechnology. 44, 335–353.
 
27.
Looijesteijn, P.J., Boels, I.C., Kleerebezem, M., Hugenholtz, J.J.A., Microbiology, E., 1999. Regulation of exopolysaccharide production by Lactococcus lactis subsp. cremoris by the sugar source. Applied and Environmental Microbiology. 65, 5003–5008. https://doi.org/10.1128/aem.65....
 
28.
Looijesteijn, P.J., Trapet, L., Vries, E., Abee, T., J, J., 2001. Physiological function of exopolysaccharides produced by Lactococcus lactis.. 64, 71–80. https://doi.org/10.1016/S0168-....
 
29.
Maser, A., Peebo, K., Vilu, R., Nahku, R.J.R.I.M., 2020. Amino acids are key substrates to Escherichia coli BW25113 for achieving high specific growth rate. Research in Microbiology. 171, 185–193. https:// doi.org/10.1016/j.resmic.2020.....
 
30.
Maye, D.J.C., Culture, Society., 2019. ‘Smart food city’: Conceptual relations between smart city planning, urban food systems and innovation theory. City, Culture and Society. 16, 18–24. https:// doi.org/10.1016/j.ccs.2017.12.....
 
31.
Mayra-Makinen, A., Bigret, M.J.F.S., Dekker, T.N.Y.M., 2004. Industrial Use and Production of Lactic Acid Bacteria, S. Salminen, A. von Wright, A. Ouwehand, et al., (Eds.), Lactic Acid Bacteria Microbi- ological and Functional Aspects. Marcel Dekker, Inc, New York, pp. 175–198.
 
32.
Mazmanian, S.K., Kasper, D.L.J.N.R.I., 2006. The love–hate relationship between bacterial polysaccharides and the host immune system. Nature Reviews Immunology. 6, 849–858. https://doi.org/10.1038/ nri1956.
 
33.
Millard, C.S., Chao, Y.P., Liao, J.C., Donnelly, M.I.J.A., Microbiology, E., 1996. Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli. Applied and Environmental Microbiology. 62, 1808–1810. https://doi.org/10.1128/aem.62....
 
34.
Muhammed, A.S., Memon, H., Baloch, S., Osman, K.A., Ali, S.A.J.P.G.C.P., 2013. Effect of Bio-organic And Inorganic Fertilizers On The Growth And Yield Of Wheat. Current Investigations in Agriculture and Current Research.
 
35.
Oh, D.-K., 2007. Tagatose: properties, applications, and biotechnological processes. Applied Microbiology and Biotechnology. 76, 1–8. https:// doi.org/10.1007/s00253-007-098....
 
36.
Ooyen, J.V., Noack, S., Bott, M., Reth, A., B, L.J., 2012. Improved L- lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity. Biotechnology & Bio- engineering. 109, 2070–2081. https://doi.org/10.1002/bit.24....
 
37.
Ottaway, P.B., 2012. The Technology of Vitamins in Food, In: 1st (Eds.); and others, (Eds.). Springer Science & Business Media, Boston, MA, p. 270. https://doi.org/10.1007/978-1-....
 
38.
Partridge, D., Lloyd, K., Rhodes, J., Walker, A., Johnstone, A., Campbell, B.J.N.B., 2019. Food additives: Assessing the impact of exposure to permitted emulsifiers on bowel and metabolic health.– introducing the FADiets study. Nutrition Bulletin. 44, 329–349. https://doi.org/10.1111/nbu.12....
 
39.
Russ, W.P., Figliuzzi, M., Stocker, C., Barrat-Charlaix, P., Socolich, M., Kast, P., Hilvert, D., Monasson, R., Cocco, S., Weigt, M.J.S., 2020. An evolution-based model for designing chorismate mutase enzymes. Science. 369, 440–445. https://doi.org/10.1126/scienc....
 
40.
Sales, A., Paulino, B.N., Pastore, G.M., Bicas, J.L., 2018. Biogeneration of aroma compounds. Current Opinion in Food Science. 19, 77–84. https://doi.org/10.1016/j.cofs....
 
41.
Stols, L., Donnelly, M.I.J.A., Microbiology, E., 1997. Production of succinic acid through overexpression of NAD(+)-dependent malic enzyme in an Escherichia coli mutant. Applied and Environmental Microbiology. 63, 2695–2701. https://doi.org/10.1128/aem.63....
 
42.
Tananuwong, K., Lertsiri, S.J.J.O.T.S.O.F., Agriculture., 2010. Changes in volatile aroma compounds of organic fragrant rice during storage under different conditions. Journal of the Science of Food and Agriculture. 90, 1590–1596. https://doi.org/10.1002/jsfa.3....
 
43.
Tas, A., Shah, A.J.T.I.F.S., Technology., 2021. The replacement of cereals by legumes in extruded snack foods: Science, technology and challenges. Trends in Food Science & Technology. 116, 701–711. https://doi.org/10.1016/j.tifs....
 
44.
Toe, C.J., Foo, H.L., Loh, T.C., Mohamad, R., Rahim, R., J, Z., 2019. Extracellular Proteolytic Activity and Amino Acid Production by Lactic Acid Bacteria Isolated from Malaysian Foods. International Journal of Molecular Sciences. 20(7), 1777. https://doi.org/10.3390/ ijms20071777.
 
45.
Tomar, N., De, R.K.J.G., 2013. Comparing methods for metabolic network analysis and an application to metabolic engineering. Gene. 521, 1–14. https://doi.org/10.1016/j.gene....
 
46.
Tzin, V., Galili, G., 2010. The biosynthetic pathways for shikimate and aromatic amino acids in Arabidopsis thaliana. Arabidopsis Book. 8, e0132. https://doi.org/10.1199/tab.01....
 
47.
Vickroy, T.W., Watson, M., Leventer, S.M., Roeske, W.R., Hanin, I., Yamamura, H.I.J.J.O.P., Therapeutics, E., 1985. Regional differences in ethylcholine mustard aziridinium ion (AF64A)-induced deficits in presynaptic cholinergic markers for the rat central nervous system. Journal of Pharmacology and Experimental Therapeutics. 235, 577– 582.
 
48.
Vilela, A., Cosme, F., Inês, A.J.F., 2020. Wine and Non-Dairy Fermented Beverages: A Novel Source of Pro-and Prebiotics. Fermentation. 6, 113. https://doi.org/10.3390/fermen....
 
49.
Wang, W., Ma, H., Zhu, Y., Ni, K., Qin, G., Tan, Z., Wang, Y., Wang, L.,.J, H., 2021. Screening of Lactic Acid Bacteria with Inhibitory Activity against ETEC K88 as Feed Additive and the Effects on Sows and Piglets. Animals. 11, 1719. https://doi.org/10.3390/ani110....
 
50.
Wang, Y.C., Yu, R.C., Chou, C.-C.J.F.M., 2002. Growth and survival of bifidobacteria and lactic acid bacteria during the fermentation and storage of cultured soymilk drinks. Food Microbiology. 19, 501–508. https://doi.org/10.1006/fmic.2....
 
51.
Wenzel, S.C., Müller, R.J.C., 2005. Recent developments towards the heterologous expression of complex bacterial natural product biosynthetic pathways. Current Opinion in Biotechnology. 16, 594– 606. https://doi.org/10.1016/j.copb....
 
52.
Wolfe, A.J.J.M., 2005. The acetate switch. Microbiology and Molecular Biology Reviews. 69, 12–50. https://doi.org/10.1128/mmbr.6....
 
53.
Yamauchi, Y., Hirasawa, T., Nishii, M., Furusawa, C., T, H.J., 2014. Enhanced acetic acid and succinic acid production under microaerobic conditions by Corynebacterium glutamicum harboring Escherichia coli transhydrogenase gene pntAB. The Journal of General and Applied Microbiology. 60, 112–118. https://doi.org/10.2323/ jgam.60.112.
 
54.
Zhang, B., Tian, W., Wang, S., Yan, X., Jia, X., Pierens, G.K., Chen, W., Ma, H., Deng, Z., J, X., 2017. Activation of Natural Products Biosynthetic Pathways via a Protein Modification Level Regulation. ACS Chemical Biology. 12, 1732–1736. https://doi.org/10.1021/ acschembio.7b00225.